Coding
Gal4(65)

Part:BBa_K4211019

Designed by: Peisong Wu   Group: iGEM22_BNUZH-China   (2022-09-19)


Gal4(65): A positive regulator of gene expression of galactose-induced genes

The Gal4 transcription factor is a positive regulator of gene expression of galactose-induced genes. This protein represents a large fungal family of transcription factors, Gal4 family, which includes over 50 members in the yeast Saccharomyces cerevisiae. Gal4(65) is a 65-amino acid residue in Gal4 protein (Gal4 residue 1-65).


Usage and Biology

We used to ligate VVD and bind to the yeast upstream activation sequence of 5×UAS under blue light irradiation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
biologyYeast
proteinGal4(65)